Pathogenic Free-Living Amebae

Immunocytologic Demonstration and Species Identification

Clyde G. CulbertsonLilly Laboratory for Clinical Research, Bureau of Laboratories, Indiana State Board of Health, 1001 West Tenth Street, Indianapolis, Indiana 46202

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Kathleen HarperLilly Laboratory for Clinical Research, Bureau of Laboratories, Indiana State Board of Health, 1001 West Tenth Street, Indianapolis, Indiana 46202

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This report describes methods for preparation, immunologic marking, and staining of amebae in formalin-fixed brain tissue, and in exudates, discharges and body fluids. The suggested procedures provide slide preparations of unknown specimens together with known amebae from cultures as controls, all on a single slide for each antiserum. This allows for cytologic studies of host cells, morphology of the amebae present, and specific immune marking of the amebae by use of immune sera and protein A Staphylococcus. Such preparations may also be used for immunoperoxidase and immunofluorescence. The need for the utilization of direct microscopic examination by the methods suggested here, or comparable ones, is discussed.

Author Notes

Retired 31 August 1983.

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