Diagnosis of Chagas' Disease in Humans Using a Biotin-3H-Avidin Radioimmunoassay

R. L. Tarleton Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109

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C. L. Schulz Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109

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M. Grögl Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109

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R. E. Kuhn Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109

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DOI:
https://doi.org/10.4269/ajtmh.1984.33.34
Volume/Issue:
Volume 33: Issue 1
Page(s):
34–40
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A radioimmunoassay (RIA) originally designed to measure antibody responses to Trypanosoma cruzi in mice was adapted for use in the immunodiagnosis of Chagas' disease in humans. The assay utilizes biotinylated antibodies and 3H-avidin as the tracer molecules, and has proven to be both safe and sensitive. Results using the RIA and those from direct agglutination and indirect fluorescent antibody tests were comparable in most cases. Using the RIA, we were able to discriminate between mice infected with T. cruzi and Trypanosoma rangeli. Also, sera from Leishmania-infected individuals do not have detectable levels of antibodies capable of binding to T. cruzi. Intact, fixed epimastigotes of T. cruzi are used as the detecting antigen in the RIA and give results comparable to those obtained with intact trypomastigotes.

Author Notes

Supported in part by a National Science Foundation Graduate Fellowship.

Copyright:
Copyright © 1984 by The American Society of Tropical Medicine and Hygiene 1984
Accepted:
01 Jun 1983
|
Published Online:
Jan 1984
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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