Identification of Hypnozoites and Tissue Schizonts of Plasmodium Vivax and P. Cynomolgi by the Immunoperoxidase Method

Frank B. CogswellDepartment of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, Laboratory Research Branch, National Hansen's Disease Center, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, New Orleans, Louisiana 70112

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Wojciech A. KrotoskiDepartment of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, Laboratory Research Branch, National Hansen's Disease Center, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, New Orleans, Louisiana 70112

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Michael R. HollingdaleDepartment of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, Laboratory Research Branch, National Hansen's Disease Center, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, New Orleans, Louisiana 70112

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Robert W. GwadzDepartment of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, Laboratory Research Branch, National Hansen's Disease Center, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, New Orleans, Louisiana 70112

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An immunoperoxidase technique was used to detect hypnozoites and liver schizonts of the primate malaria species Plasmodium vivax and P. cynomolgi bastianellii in Carnoy's-fixed sections. Anti-P. cynomolgi serum and a peroxidase-conjugated anti-monkey IgG serum rendered 7-day pre-erythrocytic forms clearly visible. The technique retains the specificity of the immunofluorescence method while having the advantage of a permanent preparation. Detection of hyponozoites by this alternative method provides further evidence for their plasmodial nature.

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