IgE Responses in Human Schistosomiasis

I. Quantitation of Specific IgE by Radioimmunoassay and Correlation of Results with Skin Test and Basophil Histamine Release

R. HussainLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Bethesda, Maryland 20205, Puerto Rico

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M. HofstetterLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Bethesda, Maryland 20205, Puerto Rico

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A. GoldstoneLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Bethesda, Maryland 20205, Puerto Rico

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W. B. KnightLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Bethesda, Maryland 20205, Puerto Rico

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E. A. OttesenLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Bethesda, Maryland 20205, Puerto Rico

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We have developed a non-competitive solid-phase radioimmunoassay (SPRIA) to quantitate human IgE antibodies to Schistosoma mansoni antigens that was capable of accurately measuring IgE antibodies in both high and low titer sera. Ninety sera from “early” or “chronic” schistosomiasis patients were studied. Both levels of parasite-specific IgE antibodies and the proportion of the total IgE response that was parasite-specific were significantly lower in “early infection” sera than in sera of patients with chronic infections. The SPRIA to adult worm antigen was compared with basophil histamine release and direct intradermal skin testing also performed with the adult antigen. Both tests showed significant correlations with the SPRIA in detecting specific anti-parasite antibody.

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