In Vitro Cultivation of the Exoerythrocytic Stage of Plasmodium Berghei in a Hepatoma Cell Line

Michael R. HollingdaleBiomedical Research Institute, 12111 Parklawn Drive, Rockville, Maryland 20852

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Pamela LelandBiomedical Research Institute, 12111 Parklawn Drive, Rockville, Maryland 20852

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Alan L. SchwartzDivision of Pediatric Hematology-Oncology, Children's Hospital Medical Center, Sidney Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115

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When inoculated with sporozoites of Plasmodium berghei, a line of hepatoma cells (HepG2-A16) derived from human liver supports the complete asexual developmental cycle of the exoerythrocytic stage. Parasites were shown to resemble parasites in vivo in hepatocytes. Subinoculation of merozoites into mice induced a red blood cell infection.

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