Author:
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 653 | 434 | 23 |
| Full Text Views | 9 | 0 | 0 |
| PDF Downloads | 7 | 0 | 0 |
Development of an Enzyme-Linked Immunosorbent Assay using Arabinomannan from Mycobacterium Smegmatis: A Potentially Useful Screening Test for the Diagnosis of Incubating Leprosy
Richard A. Miller
Richard A. Miller
Immunology Research Laboratory, Seattle Public Health Hospital, and Department of Medicine and Pathobiology, University of Washington, Department of Biochemistry, Faculty of Medicine, University of Peradeniya, Seattle, Washington 98195, Sri Lanka
Search for other papers by Richard A. Miller in
Current site
Google Scholar
PubMed
Search for other papers by Richard A. Miller in
Current site
Google Scholar
PubMed
Senarath Dissanayake
Senarath Dissanayake
Immunology Research Laboratory, Seattle Public Health Hospital, and Department of Medicine and Pathobiology, University of Washington, Department of Biochemistry, Faculty of Medicine, University of Peradeniya, Seattle, Washington 98195, Sri Lanka
Search for other papers by Senarath Dissanayake in
Current site
Google Scholar
PubMed
Search for other papers by Senarath Dissanayake in
Current site
Google Scholar
PubMed
Thomas M. Buchanan
Thomas M. Buchanan
Immunology Research Laboratory, Seattle Public Health Hospital, and Department of Medicine and Pathobiology, University of Washington, Department of Biochemistry, Faculty of Medicine, University of Peradeniya, Seattle, Washington 98195, Sri Lanka
Search for other papers by Thomas M. Buchanan in
Current site
Google Scholar
PubMed
Search for other papers by Thomas M. Buchanan in
Current site
Google Scholar
PubMed
A carbohydrate antigen composed predominantly of arabinomannan has been purified from Mycobacterium smegmatis and used in an enzyme-linked immunosorbent assay to detect anti-mycobacterial antibodies in human sera. Sera from 117 controls, 25 tuberculosis patients, 124 leprosy patients and 256 household contacts of leprosy patients were tested. When compared with the control group, 56% of tuberculosis patients, 27% of patients with tuberculoid leprosy, 77% of borderline leprosy cases, and 95% of patients with lepromatous leprosy had elevated titers. Nine percent of the household contact group had abnormally high levels of antibody. The relevance of these findings to the serodiagnosis of incubating leprosy and the management of household contacts of leprosy patients is discussed.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 653 | 434 | 23 |
| Full Text Views | 9 | 0 | 0 |
| PDF Downloads | 7 | 0 | 0 |