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Induced Division Synchrony in Entamoeba Histolytica
Effects of Hydroxyurea and Serum Deprivation
Christopher J. Austin
Christopher J. Austin
Department of Biochemistry and Department of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, Louisiana 70112
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Lionel G. Warren
Lionel G. Warren
Department of Biochemistry and Department of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, Louisiana 70112
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Synchronous growth of axenic Entamoeba histolytica was induced by nutritional deprivation and/or hydroxyurea. Axenic amebae maintained in 2% serum culture medium for 17–25 hours and returned to complete TP-S-1 (10% serum) medium showed a single synchronous cycle with a mean division time of 8.8 hours and a synchrony index of 0.47. Exposure to 10 mM hydroxyurea for 20–26 hours induced a single synchronous cycle with a division time of 3.0 hours and a synchrony index of 0.85. Hydroxyurea was toxic to 56% of the cells in the culture. The control generation time in TP-S-1 medium was 14.8 hours. Cells maintained for 44 hours in a nucleotide-deficient medium followed by a 10 mM hydroxyurea treatment exhibited two synchronous cycles. The first cycle had a generation time of 5.0 hours, a division time of 2.2 hours, and a synchrony index of 0.84.
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