Enzyme-Linked Immunosorbent Assay (Elisa) for the Detection of Antibody to Cysticerci of Taenia Solium

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  • * Laboratory of Central Nervous System Studies, NINCDS, National Institutes of Health, Bethesda, Maryland 20205
  • | Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Honolulu, Hawaii 96816
  • | Public Health Department, Jayapura, Irian Jaya, Indonesia
  • | § Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205
  • | Department of Neuropathology, Instituto Nacional de Neurologica y Neurocirugia, Mexico City, Mexico
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An enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to cysticerci of Taenia solium has been developed that employs a pork muscle antigen control for the cysticercus test antigen, somewhat improving the serological distinction between infected and uninfected subjects. Serum antibody to cysticercus was detected in 79% of classical neurocysticercosis patients from Mexico, and in 61% of a group of cysticercosis patients with an unusually rapid invasion of the central nervous system in an endemic focus of disease in Irian Jaya. Antibody was absent in a group of healthy American laboratory personnel, and in residents of a non-endemic region of Papua New Guinea. Additional tests on sera from patients with other parasitic diseases showed that cross-reactivity may occur in some patients with schistosomiasis, echinococcosis, and possibly angiostrongyliasis; however, these parasites are not known to cause human infection in Irian Jaya.