Immunosorbent Assays for Diagnosis of Crimean-Congo Hemorrhagic Fever (CCHF)

M. A. Donets Institute of Poliomyelitis and Viral Encephalitides of the USSR Academy of Medical Sciences, 142782 Moscow, USSR

Search for other papers by M. A. Donets in
Current site
Google Scholar
PubMed Close
,
G. V. Rezapkin Institute of Poliomyelitis and Viral Encephalitides of the USSR Academy of Medical Sciences, 142782 Moscow, USSR

Search for other papers by G. V. Rezapkin in
Current site
Google Scholar
PubMed Close
,
A. P. Ivanov Institute of Poliomyelitis and Viral Encephalitides of the USSR Academy of Medical Sciences, 142782 Moscow, USSR

Search for other papers by A. P. Ivanov in
Current site
Google Scholar
PubMed Close
, and
E. A. Tkachenko Institute of Poliomyelitis and Viral Encephalitides of the USSR Academy of Medical Sciences, 142782 Moscow, USSR

Search for other papers by E. A. Tkachenko in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1982.31.156
Volume/Issue:
Volume 31: Issue 1
Page(s):
156–162
Restricted access
Get Permissions

Utilization of direct and indirect enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (SPRIA) for the diagnosis of Crimean-Congo hemorrhagic fever (CCHF) allows the detection of low amounts of infectious virus (2 log LD50) or inactivated antigen and antibody to CCHF within 5–6 hours. These methods were shown to be more sensitive, specific, rapid, and reproducible than the complement-fixation test, immunofluorescence, hemagglutination, or radial diffusion in gel. The experimental design of ELISA and SPRIA developed for CCHF may be used successfully for the detection of the other members of the Bunyaviridae family.

Author Notes

Copyright:
Copyright © 1982 by The American Society of Tropical Medicine and Hygiene 1982
Accepted:
11 May 1981
|
Published Online:
Jan 1982
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 665 315 47
Full Text Views 16 2 0
PDF Downloads 14 6 0
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save