Factors Affecting Syncytial Development in Aedes Pseudoscutellaris Cells by Dengue Viruses

Goro Kuno San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, GPO Box 4532, San Juan,, Puerto Rico

Search for other papers by Goro Kuno in
Current site
Google Scholar
PubMed Close
,
Chester G. Moore San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, GPO Box 4532, San Juan,, Puerto Rico

Search for other papers by Chester G. Moore in
Current site
Google Scholar
PubMed Close
, and
Gladys E. Sather San Juan Laboratories, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, GPO Box 4532, San Juan,, Puerto Rico

Search for other papers by Gladys E. Sather in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1981.30.870
Volume/Issue:
Volume 30: Issue 4
Page(s):
870–875
Restricted access
Get Permissions

Several factors that were suspected of affecting the development of syncytia in cultured Aedes pseudoscutellaris cells inoculated with dengue viruses were studied. The results indicated that fresh media (less than 1 week old), low cell density at inoculation (2.8 × 105 cells/cm2), and low cell passage level (less than 52 passages) favored the development of syncytia. All three types (1, 2, and 3) of dengue viruses tested could be isolated from human sera by Aedes pseudoscutellaris cell culture by using syncytial development as an indicator, but the isolation rate was lower than that obtained by using intrathoracically inoculated Aedes aegypti mosquitoes.
Copyright:
Copyright © 1981 by The American Society of Tropical Medicine and Hygiene 1981
Accepted:
10 Jan 1981
|
Published Online:
Jul 1981
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 240 129 2
Full Text Views 2 0 0
PDF Downloads 0 0 0
 
Membership Banner
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save