Cell-Mediated Cytotoxicity to Trypanosoma Cruzi

II. Antibody-Dependent Killing of Bloodstream Forms by Mouse Eosinophils and Neutrophils

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  • Departments of Medicine and Pathology, Harvard Medical School, Robert B. Brigham Hospital Division of the Brigham and Women's Hospital, Boston, Massachusetts 02115

Mouse mononuclear cells, neutrophils, and eosinophils were tested for their capacity to mediate antibody-dependent cytotoxicity against bloodstream trypomastigotes of Trypanosoma cruzi. Granulocyte populations were found to be far more effective than nonadherent mononuclear cells in an in vitro assay in which the number of motile parasites was measured. Eosinophils and neutrophils were observed to be equally efficient on a cell-per-cell basis in killing the trypomastigotes. These results were verified in parallel experiments in which trypomastigotes, after incubation with antibody and effector cells, were reinjected into susceptible mice and the survival of the animals was determined.

Author Notes

Supported by the Special Programme for Research and Training in Tropical Diseases, World Health Organization.

Recipient of a Career Development Award in Geographic Medicine from the Rockefeller Foundation.