Separation of Stages of Plasmodium Falciparum-Infected Cells by means of a Fluorescence-Activated Cell Sorter

Graham V. BrownThe Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia

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Francis L. BattyeThe Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia

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Russell J. HowardThe Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia

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Plasmodium falciparum parasites from long-term in vitro culture have been labeled with the DNA-binding dye Hoechst 33258. After labeling, parasitized cells have been successfully analyzed and sorted, using a fluorescence-activated cell sorter, into populations of uninfected, singly infected, and multiply infected cells.

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Present address: Malaria Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20205.

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