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Detection of Circulating Antibodies in Cutaneous Leishmaniasis by Enzyme-Linked Immunosorbent Assay (ELISA)
Jean Roffi
Jean Roffi
Institute Pasteur, B.P. 220, Dakar, Sénégal
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Jean-Pierre Dedet
Jean-Pierre Dedet
Institute Pasteur, B.P. 220, Dakar, Sénégal
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Philippe Desjeux
Philippe Desjeux
Institute Pasteur, B.P. 220, Dakar, Sénégal
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Marie-Thérèse Garré
Marie-Thérèse Garré
Institute Pasteur, B.P. 220, Dakar, Sénégal
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An immunoenzymatic diagnostic technique applicable to cutaneous leishmaniasis is described. The antigen used (Leishmania tropica major) was equally useful in diagnosing visceral and mucocutaneous forms of the disease. The criteria for positivity were defined by using groups of negative controls, and the specificity of the reaction was evaluated by using sera from patients with various diseases. Among these, sera from patients with lepromatous leprosy, tuberculosis, or African trypanosomiasis strongly cross-reacted with leishmania antigen. Examining serial dilutions of the sera facilitated the interpretation of the results and eliminated a significant percentage of false positives.
Author Notes
Present address: Unité de Parasitologie Expérimentale, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 521 | 271 | 14 |
| Full Text Views | 15 | 2 | 1 |
| PDF Downloads | 14 | 2 | 0 |