Evaluation of the Micro Enzyme-Linked Immunosorbent Assay for Antibodies to Trypanosoma Cruzi

Harrison C. SpencerBureau of Tropical Diseases and Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

Search for other papers by Harrison C. Spencer in
Current site
Google Scholar
PubMed
Close
,
Dorothy S. AllainBureau of Tropical Diseases and Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

Search for other papers by Dorothy S. Allain in
Current site
Google Scholar
PubMed
Close
,
Alexander J. SulzerBureau of Tropical Diseases and Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

Search for other papers by Alexander J. Sulzer in
Current site
Google Scholar
PubMed
Close
, and
William E. CollinsBureau of Tropical Diseases and Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

Search for other papers by William E. Collins in
Current site
Google Scholar
PubMed
Close
Restricted access

A micro enzyme-linked immunosorbent assay (ELISA) for antibodies to Trypanosoma cruzi was evaluated and the results obtained by ELISA were compared with those obtained by the complement fixation test (CF) and indirect fluorescent antibody test (IFA). Fifty sera collected from residents of the southeastern United States all had reciprocal ELISA titers ⩽320. Similarly, serum samples from 17 patients with T. cruzi infection proven by xenodiagnosis had reciprocal ELISA titers of ⩾1,280. Specimens from 302 El Salvador Army recruits were tested by ELISA, IFA, and CF. Excellent correlation was observed between results obtained by the three serologic tests; 62.9% of the samples were negative by each of the three tests and 24.5% were positive by all. Overall, 29.5% of the sera were positive for antibodies to T. cruzi by ELISA, 29.5% by IFA, and 31.5% by CF. The data suggest that the micro ELISA is a promising serologic test for measuring antibodies to T. cruzi in individuals and in populations.

Save