Purification of Giardia Muris Trophozoites by Using Nylon Fiber Columns

John S. Andrews Jr.Divisions of Geographic Medicine and Gastroenterology, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals, Cleveland, Ohio 44106

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Jerrold J. EllnerDivisions of Geographic Medicine and Gastroenterology, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals, Cleveland, Ohio 44106

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David P. StevensDivisions of Geographic Medicine and Gastroenterology, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals, Cleveland, Ohio 44106

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A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 × 106 viable purified trophozoites per infected mouse.

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