Antigenic Analyses of Two Axenized Strains of Entamoeba Histolytica by Two-Dimensional Immunoelectrophoresis

Shu Mei ChangU.S. Naval Medical Research Unit No. 2, Department of Life Sciences, Indiana State University, Taipei, Taiwan, Republic of China

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Chun Mei LinU.S. Naval Medical Research Unit No. 2, Department of Life Sciences, Indiana State University, Taipei, Taiwan, Republic of China

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Donald G. DusanicU.S. Naval Medical Research Unit No. 2, Department of Life Sciences, Indiana State University, Taipei, Taiwan, Republic of China

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John H. CrossU.S. Naval Medical Research Unit No. 2, Department of Life Sciences, Indiana State University, Taipei, Taiwan, Republic of China

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Antigens in extracts of two strains of Entamoeba histolytica (HT-31 and HK-9) were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, respectively. Cross-reactions between heterologous systems showed a large number of shared antigens between the two strains. Extracts were fractionated by Sephadex G-200 chromatography. Of the fractions obtained, fraction II of both strains was found to have the most reactive antigenic components. Precipitin patterns of fraction II from HT-31 and HK-9 with sera from patients showed similar 2D-IEP profiles and suggested that in this fraction antigens common to both strains exist which elicit antibody in individuals with E. histolytica infections.

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