Cultured Plasmodium Falciparum Used as Antigen in a Malaria Indirect Fluorescent Antibody Test

Cynthia L. Hall Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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J. David Haynes Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Jeffrey D. Chulay Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Carter L. Diggs Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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DOI:
https://doi.org/10.4269/ajtmh.1978.27.849
Volume/Issue:
Volume 27: Issue 5
Page(s):
849–852
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Human sera obtained from persons infected with Plasmodium falciparum were tested by a standard indirect fluorescent antibody (IFA) technique using antigen obtained from long term in vitro cultures of two strains of P. falciparum, and antibody in high titer was reproducibly detected. Sera from uninfected persons had undetectable or very low titers of antibody. The use of cultured parasites offers a convenient, stable source of antigens from different P. falciparum strains without requiring their adaptation to primates. Differences observed in IFA titers obtained by reacting immune serum with two different P. falciparum strains suggests the need for further evaluation of strain specificity.

Author Notes

Copyright:
Copyright © 1978 by The American Society of Tropical Medicine and Hygiene 1978
Accepted:
25 Feb 1978
|
Published Online:
Sep 1978
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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