Growth of Babesia Bovis in Bovine Erythrocyte Cultures

Elton E. ErpDepartment of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

Search for other papers by Elton E. Erp in
Current site
Google Scholar
PubMed
Close
,
Sally M. GravelyDepartment of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

Search for other papers by Sally M. Gravely in
Current site
Google Scholar
PubMed
Close
,
Ronald D. SmithDepartment of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

Search for other papers by Ronald D. Smith in
Current site
Google Scholar
PubMed
Close
,
Miodrag RisticDepartment of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

Search for other papers by Miodrag Ristic in
Current site
Google Scholar
PubMed
Close
,
B. Miguel OsornoDepartment of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

Search for other papers by B. Miguel Osorno in
Current site
Google Scholar
PubMed
Close
, and
C. A. CarsonDepartment of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

Search for other papers by C. A. Carson in
Current site
Google Scholar
PubMed
Close
Restricted access

Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37°C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals. Persistent multiplication of the parasite in a short series of subcultures suggests the feasibility of this approach for continuous culture.

Save