Purification of a Specific Antigen of Trypanosoma Cruzi from Culture Forms

Daniel Afchain Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Laboratoire de Parasitologie, U.E.R. de Pharmacie, Lille, France

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Jeanine Fruit Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Laboratoire de Parasitologie, U.E.R. de Pharmacie, Lille, France

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Luis Yarzabal Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Laboratoire de Parasitologie, U.E.R. de Pharmacie, Lille, France

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André Capron Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Laboratoire de Parasitologie, U.E.R. de Pharmacie, Lille, France

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DOI:
https://doi.org/10.4269/ajtmh.1978.27.478
Volume/Issue:
Volume 27: Issue 3
Page(s):
478–482
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A monospecific rabbit antiserum was prepared against a specific component of Trypanosoma cruzi epimastigote culture forms (designated antigen 5). Rabbits were immunized intradermally in 30–50 sites with the zone of agarose gel in which the component 5:anti-5 complex was visualized after two-dimensional immunoelectrophoresis. With this appropriate antiserum and the total epimastigote soluble extracts, the specific component of T. cruzi was purified by immunoadsorption. Purity and specificity controls of the final product were carried out by prolonged immunization of rabbits, and then by immunoelectrophoretic and two-dimensional electrophoretic analyses.

Author Notes

Copyright:
Copyright © 1978 by The American Society of Tropical Medicine and Hygiene 1978
Accepted:
03 Dec 1977
|
Published Online:
May 1978
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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