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Olifantsvlei (OLI)
Strain: SA-AR5133
Information from: B. M. McIntosh, South African Institute for Medical Research, P.O. Box 1038, Johannesburg 2001, 5 August 1975.
Antigenic group: Olifantsvlei (Bunyamwera Supergroup).
Original source: Isolated by B. M. McIntosh at S.A.I.M.R., Johannesburg, from 250 adult female Culex pipiens collected 8 January 1963 with solid CO2 baited traps at Olifantsvlei, Johannesburg, S. Africa, Lat. 26°S, Long. 28°E. Habitat: Grassland plateau, 1,500 m in elevation, with riverine reed bed at ground level. Storage: Frozen on dry ice.
Method of isolation in laboratory: Mosquitoes ground in 0.75% bovine albumin solution. Inoculated 0.02 ml ic into 2-day-old mice.
Validity of isolation: Reisolation not tried, but AR5 133 immunologically distinct from other viruses in laboratory; other isolations from mosquitoes made elsewhere in Africa.