Single Inoculation Immune Hamster Sera for Typing California Group Arboviruses by the Complement-Fixation Test

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  • Department of Epidemiology and Public Health, Yale School of Medicine, New Haven, Connecticut 06510

Eight reference California group viruses of North America were typed using sera of immune hamsters bled 21 days after a single inoculation. The complement-fixation test reactions were relatively specific, although only 2-fold differences were observed reciprocally with the closely-related La Crosse and snowshoe hare viruses. Hamster serum taken 10 days post inoculation was more specific than a 21-day serum. Specificity after second inoculation was lost with some antigens. Jamestown Canyon and South River viruses were identical by complement-fixation test and showed minor differences in the plaque reduction neutralization test.

Author Notes

Present address: Department of Entomology, University of Maryland, College Park, Maryland 20742.