A Simple Technique for the Detection of Dengue Antigen in Mosquitoes by Immunofluorescence

Timothy T. KuberskiPacific Research Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Honolulu, Hawaii 96806

Search for other papers by Timothy T. Kuberski in
Current site
Google Scholar
PubMed
Close
and
Leon RosenPacific Research Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Honolulu, Hawaii 96806

Search for other papers by Leon Rosen in
Current site
Google Scholar
PubMed
Close
Restricted access

Dengue antigen could be demonstrated by a direct fluorescent antibody technique in simple head squashes of either male or female Aedes albopictus mosquitoes infected with any of the four dengue serotypes. Dengue viruses can be isolated rapidly and simply by combining this technique with that of parenteral inoculation of mosquitoes with test material.

Save