The Effects of Lampit (Bayer 2502) on the Interaction of Trypanosoma Cruzi with Vertebrate Cells in Vitro

James A. DvorakLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

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Christine L. HoweLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

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The effects of 3-methyl-4-(5′-nitrofurfurylidene-amino)-tetrahydro-4H-1,4-thiazine-1,1-dioxide [Lampit, Bayer 2502] on the intracellular cycle of Trypanosoma cruzi were studied with an in vitro steady-state culture system that permitted the continuous analysis of individual host cell-parasite interactions. Lampit concentrations of 10-4 and 10-5 M significantly affected the ability of trypomastigotes to penetrate vertebrate cells. Lampit concentrations above 10-4 M were toxic to the host cell population and Lampit concentrations below 10-5 M did not affect the ability of trypomastigotes to penetrate vertebrate cells. Lampit concentrations of 10-5 M or greater completely inhibited the intracellular cycle of T. cruzi whereas Lampit concentrations of 10-7 M or less had no effect on the intracellular cycle. Continuous perfusion with 10-6 M Lampit resulted in a linear increase in intracellular parasite reproduction time. A stable strain of T. cruzi was produced in vitro that was resistant to a 100-fold greater concentration of Lampit than the parent strain.

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