A Note on the Use of Trypsinized Human Group O Erythrocytes in Arbovirus Titrations

Kennedy F. ShortridgeDepartment of Microbiology, University of Hong Kong, Pathology Building, Queen Mary Hospital Compound, Hong Kong

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Hu Li-YuDepartment of Microbiology, University of Hong Kong, Pathology Building, Queen Mary Hospital Compound, Hong Kong

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Twenty-two different arbovirus antigens derived from five antigenic groups were examined for their ability to agglutinate normal and trypsinized human group O eryth-rocytes. Trypsinization rendered the cells susceptible to agglutination by 18 viruses, chiefly in the pH 5.85 to 6.2 range, yielding hemagglutinin titers comparable with those obtained with gander cells at their optimal pH.

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