Evaluation of Whole and Purified Hydatid Fluid Antigens in the Diagnosis of Human Hydatidosis by the Immunoelectrophoresis Test

V. M. Varela-Díaz Pan American Zoonoses Center, Pan American Health Organization, World Health Organization, Laboratorio de Inmunología Parasitaria, Hospital de Clínicas, Facultad de Medicina, Casilla 23, Ramos Mejía, Argentina

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J. A. Guisantes Pan American Zoonoses Center, Pan American Health Organization, World Health Organization, Laboratorio de Inmunología Parasitaria, Hospital de Clínicas, Facultad de Medicina, Casilla 23, Ramos Mejía, Argentina

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M. I. Ricardes Pan American Zoonoses Center, Pan American Health Organization, World Health Organization, Laboratorio de Inmunología Parasitaria, Hospital de Clínicas, Facultad de Medicina, Casilla 23, Ramos Mejía, Argentina

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L. A. Yarzábal Pan American Zoonoses Center, Pan American Health Organization, World Health Organization, Laboratorio de Inmunología Parasitaria, Hospital de Clínicas, Facultad de Medicina, Casilla 23, Ramos Mejía, Argentina

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E. A. Coltorti Pan American Zoonoses Center, Pan American Health Organization, World Health Organization, Laboratorio de Inmunología Parasitaria, Hospital de Clínicas, Facultad de Medicina, Casilla 23, Ramos Mejía, Argentina

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DOI:
https://doi.org/10.4269/ajtmh.1975.24.298
Volume/Issue:
Volume 24: Issue 2
Page(s):
298–303
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Whole sheep hydatid cyst fluid (WHF) and purified hydatid fluid antigens (PHF) were evaluated in the immunoelectrophoresis (IEP) test for the diagnosis of human hydatid disease. A higher sensitivity was obtained using the PHF than with the WHF antigen but false positive results were observed with the former in 22.4% of the 102 non-hydatid sera examined. Using the presence of arc 5 as the criterion of positivity however, no false positives were observed in non-hydatid sera with the WHF antigen. It is postulated that the number of bands other than arc 5 may be of value in the diagnosis of hydatid disease by the IEP test, in cases where this diagnostic arc is absent.

Author Notes

Fellow, Pan American Health Organization.

Short-term consultant, Pan American Health Organization.

Copyright:
Copyright © 1975 by The American Society of Tropical Medicine and Hygiene 1975
Accepted:
13 Jul 1974
|
Published Online:
Mar 1975
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637

Diagnostic Procedures and Reagents

Technics for the Laboratory Diagnosis and Control of the Communicable Diseases, American Public Health Association, Ed., pp. v plus 589, 6 plates in color, cloth, New York, Am. Pub. Health Assoc. Inc., 1950, $6.00

An Introduction to Medical Protozoology

With Chapters on the Spirochaetes and on Laboratory Methods. By Robert Knowles, B.A., M.R.C.S., L.R.C.P., Lt. Col., Indian Medical Service, Calcutta. Thacker, Spink and Company. 1928. Pages I–XII, 1–887

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Chas. F. Craig
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