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A Microprecipitation Test for Rapid Detection and Identification of Venezuelan, Eastern and Western Equine Encephalomyelitis Viruses
Neil H. Levitt
Neil H. Levitt
U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701
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Helen V. Miller
Helen V. Miller
U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701
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Carl E. Pedersen Jr.
Carl E. Pedersen Jr.
U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701
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Gerald A. Eddy
Gerald A. Eddy
U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701
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The development of a new diagnostic procedure for the identification of Venezuelan, eastern and western equine encephalomyelitis (VEE, EEE, WEE) viruses is described. The procedure utilizes virus precipitation with reference fluorescein-conjugated gamma globulin, followed by cellulose acetate electrophoresis. Clinical specimens containing varying concentrations of virus yielded, in primary duck embryo cell culture, sufficient virus for detection within 22 to 44 hours. Identification of VEE, EEE and WEE virus in specimens was accomplished by microprecipitation within this time. In contrast to conventional identification methods, our procedure eliminates the costs of utilizing laboratory animals and considerably reduces the time required for virus identification.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 314 | 94 | 10 |
| Full Text Views | 9 | 0 | 0 |
| PDF Downloads | 2 | 0 | 0 |