A Microprecipitation Test for Rapid Detection and Identification of Venezuelan, Eastern and Western Equine Encephalomyelitis Viruses

Neil H. LevittU. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701

Search for other papers by Neil H. Levitt in
Current site
Google Scholar
PubMed
Close
,
Helen V. MillerU. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701

Search for other papers by Helen V. Miller in
Current site
Google Scholar
PubMed
Close
,
Carl E. Pedersen Jr.U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701

Search for other papers by Carl E. Pedersen Jr. in
Current site
Google Scholar
PubMed
Close
, and
Gerald A. EddyU. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701

Search for other papers by Gerald A. Eddy in
Current site
Google Scholar
PubMed
Close
Restricted access

The development of a new diagnostic procedure for the identification of Venezuelan, eastern and western equine encephalomyelitis (VEE, EEE, WEE) viruses is described. The procedure utilizes virus precipitation with reference fluorescein-conjugated gamma globulin, followed by cellulose acetate electrophoresis. Clinical specimens containing varying concentrations of virus yielded, in primary duck embryo cell culture, sufficient virus for detection within 22 to 44 hours. Identification of VEE, EEE and WEE virus in specimens was accomplished by microprecipitation within this time. In contrast to conventional identification methods, our procedure eliminates the costs of utilizing laboratory animals and considerably reduces the time required for virus identification.

Save