Modified Antigens in the Indirect Immunofluorescence Test for Schistosomiasis

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  • Center for Disease Control, Public Health Service, U. S. Department of Health, Education and Welfare, Atlanta, Georgia

Frozen sections of Schistosoma mansoni adults and cercariae were prepared for use as slide antigens in the indirect immunofluorescence test for schistosomiasis. Unfixed adults embedded in Tissue-Tek O.C.T. medium for frozen sectioning gave the best combination of sensitivity and specificity. Cercarial antigens reacted intensely with Trichinella anti-sera. Extensive evaluation of the frozen S. mansoni and S. haematobium adult antigens indicated that the sensitivities were 91.7% and 85.4%, respectively; specificities were 95.8% and 96.9%, respectively. Within-run reproducibility within one twofold dilution was 93.7% and 97.9%, respectively. Determination of infecting species by serology was not possible.