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Colonized Culiseta melanura were infected with eastern equine encephalitis (EEE) virus by feeding on viremic 1-day-old chicks. The approximate minimal mosquito infective dose was 104 baby mouse LD50/0.02 ml of blood, the approximate 50% dose was 105, and the approximate 90% dose was 106 LD50/0.02 ml. Over 85% of infected mosquitoes transmitted EEE virus to baby chicks after a 2-week extrinsic incubation.
Present address: New York State Department of Health, 1475 Winton Road North, Rochester, New York 14609.