Experimental Infections with African Trypanosomes

I. Quantitation of Trypanosoma rhodesiense by Electronic Particle Counting

Larry K. MartinDepartment of Medical Zoology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

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Elvio H. SadunDepartment of Medical Zoology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

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Gene D. IngramDepartment of Medical Zoology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

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An electronic particle counting technique was tested for counting Trypanosoma rhodesiense in clear suspensions from DEAE cellulose column filtration, and the counts were compared to hemacytometer counts of trypanosomes in the blood and in clear suspensions. The estimated number of trypanosomes per ml of blood was approximately the same whether obtained by particle counting or by hemacytometer technique. The electronic particle counts required less time to perform, were more reproducible, and permitted the detection of trypanosomes when there were too few for disclosure by the hemacytometer method.

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