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Interaction and Transformation of Leishmania Donovani within in Vitro Cultured Cells
An Electron Microscopical Study
H. James Akiyama
H. James Akiyama
Department of Biological Sciences, Microbiology Area, California State University, San Jose, San Jose, California 95114
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Nancy K. McQuillen
Nancy K. McQuillen
Department of Biological Sciences, Microbiology Area, California State University, San Jose, San Jose, California 95114
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The interaction and subsequent transformation of Leishmania donovani within viable in vitro cell culture preparations were investigated using the electron microscope. Observed ultrastructural changes of the parasite during its transformation process corresponded with results reported by previous investigators. In the interaction of the parasite with hamster peritoneal macrophages, some of the parasites appeared to be degraded within vacuoles of the macrophage, whereas they appeared to be able to survive within similar vacuoles of the dog sarcoma 503 cell line.
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