Use of Stable Sensitized Cells in Indirect Microhemagglutination Test for Malaria

David C. FarshyParasitology Section, Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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Irving G. KaganParasitology Section, Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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Human “O” erythrocytes were stabilized with pyruvic aldehyde, tanned, and fixed with glutaraldehyde. These cells were sensitized with antigenic preparations of Plasmodium knowlesi and were used in the indirect microhemagglutination test with serum samples from human malaria cases and negative controls. The cells did not lose sensitivity after 3 months of storage. The practical application of these cells in seroepidemiological studies of malaria is pointed out.

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