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Apparently, nonspecific inhibitors of Western and Venezuelan equine encephalitis viruses were found in cattle sera from Hawaii by plaque-reduction neutralization (N) tests in avian cell cultures and by hemagglutination-inhibition (HI) tests. Yet the islands were presumably free of these viruses in 1964 to 1966 when the sera were collected, and no inhibitors were detected in sera from Hawaiian goats and pigs and in more than 98 percent of sheep sera. Specific HI and N, but not complement-fixing, antibodies, developed in the serum of one cow sequentially inoculated with Sagiyama, Western and Eastern equine encephalomyelitis viruses of group A.
Present address: Department of Hygiene, University of Tokyo Medical School, Tokyo, Japan.