Fourteen strains of Venezuelan encephalitis virus were isolated from Culex (Melanoconion) iolambdis, Culex (Culex) coronator, Culex (C.) thriambus, Culex (M.) opisthopus, Deinocerites pseudes, Haemogogus mesodentatus, and Wyeomyia mitchelli at Sontecomapan, Veracruz, during July and August 1963 and 1964, and from Aedes scapularis from Minatitlan, Veracruz, during August 1964. These isolations were from mosquitoes caught in light traps or animal-baited traps where feedback of virus to mosquitoes from viremic animal bait was impossible or unlikely. Eighteen additional strains came from these Culex and Haemogogus species under conditions where virus feedback was possible. Nineteen strains of VE virus were recovered from five other species (five genera) only under circumstances where virus feedback from viremic animal bait was possible. Even the most conservative interpretation of these results indicated that VE virus in Veracruz, México, infected several mosquito species and genera, and thus probably has multiple mosquito vectors in that region.