Susceptibility of Tissue Cultures of Minced Aedes Aegypti Pupae and Adults to Infection with Venezuelan Equine Encephalomyelitis Virus

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  • Biological Sciences Laboratory, Fort Detrick, Frederick, Maryland 21701

The Trinidad strain of Venezuelan equine encephalomyelitis virus was propagated successfully in tissue cultures of germ-free, minced Aedes aegypti pupae. Peak virus titers of 5.0 to 6.0 log10 plaque-forming units per ml were reached within 3 to 4 days after cultures were infected. Five serial passages caused no detectable changes in either mouse virulence or plaque-size characteristics of the virus. An attenuated virus strain derived from the Trinidad strain did not multiply in these cultures, and neither virus strain grew in tissue cultures of germ-free minced adult mosquitoes.