Physical Separation of Three Soluble Malarial Antigens from the Serum of Chickens Infected with Plasmodium Gallinaceum

J. D. Lykins Department of Veterinary Pathology and Hygiene and the Department of Microbiology, University of Illinois, Urbana, Illinois 61801

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A. R. Smith Department of Veterinary Pathology and Hygiene and the Department of Microbiology, University of Illinois, Urbana, Illinois 61801

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E. W. Voss Department of Veterinary Pathology and Hygiene and the Department of Microbiology, University of Illinois, Urbana, Illinois 61801

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M. Ristic Department of Veterinary Pathology and Hygiene and the Department of Microbiology, University of Illinois, Urbana, Illinois 61801

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DOI:
https://doi.org/10.4269/ajtmh.1971.20.394
Volume/Issue:
Volume 20: Issue 3
Page(s):
394–401
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Three precipitating serum soluble antigens (SAg 1, SAg 2, SAg 3) were demonstrated in the serum of chickens that had been injected with Plasmodium gallinaceum 72 to 80 hours previously. These antigens were detected when the chickens were tested by double diffusion in gel against an antiserum obtained from chickens that survived the infection. The three antigens showed a molecular weight relationship of SAg 1 > SAg 2 > SAg 3 based on their diffusion in gel and elution from Sephadex G-200. They were selectively precipitated by specified concentrations of sodium sulfate and were partially resolved from DEAE-cellulose by increasing molar concentrations of phosphate buffer. SAg 1 was antigenically unrelated to SAg 2 and SAg 3. SAg 2 appeared to contain lipid, as it was precipitated by dextran sulfate.
Copyright:
Copyright © 1971, By The American Society of Tropical Medicine and Hygiene 1971
Accepted:
11 Nov 1970
|
Published Online:
May 1971
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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