Isolations of Nepuyo Virus Strains from Honduras, 1967

C. H. Calisher Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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W. A. Chappell Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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K. S. C. Maness Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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R. D. Lord Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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W. D. Sudia Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

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Three viruses, two from bats and one from mosquitoes, were isolated from specimens field-collected in Honduras in 1967. Sodium deoxycholate sensitivity, pathological findings, temperature stability, size, and host spectrum by route and age suggested membership in the Bunyamwera supergroup. Hemagglutination-inhibition, complement-fixation, and neutralization testing with reagents prepared to 34 arboviruses demonstrated that the three strains belong to Group C and are most closely related to Nepuyo virus. There was serological evidence that man and horses had been infected with these viruses.

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