By H. J. Bensted, W. Bulloch, L. Dudgeon, A. G. Gardner, E. D. W. Greig, D. Harvey, W. F. Harvey, T. J. Mackie, R. A. O'Brien, H. M. Perry, H. Scutze, P. Bruce White, W. J. Wilson. London, 1929. His Majesty's Stationery Office. Pp. 1–482
by A. Trevor Willis, M.D., B.S. (Melb.), Ph.D. (Leeds), M.C.Path., M.C.P.A., Reader in Microbiology, Monash University, formerly Lecturer in Bacteriology, University of Leeds. xiv + 234 pages, illustrated, second edition. Butterworth Inc., Washington. 1965. $8.50
Information from: Robert P. Scrivani, School of Public Health, University of California, Berkeley, California, U.S.A., 15 January 1968.
Antigenic group: Bunyamwera.
Original source: Isolated by Robert P. Scrivani at the School of Public Health, University of California, from a pool of 50 female adult Culex tarsalis collected on 24 and 25 July 1962 in a Dry Ice-baited trap at Kern County, California, Lat. 36° 30′ N, Long. 119° W. Habitat: Farmyard. Storage: Specimens stored in a sealed glass tube at -65°F until tested.
Method of isolation in laboratory: Ground mosquito suspension inoculated into primary hamster kidney-cell cultures on 1 August 1962. Maintenance medium 0.5% lactalbumin hydrolysate in Earle's BSS with 3% calf serum and 4 mg glucose per ml. CPE shown.
Validity of isolation: Virus reisolated. No similar virus in laboratory.
Properties of virus: Physical: No data. Chemical: No data. Resistance to chemicals: Sensitive to 1:1,000 sodium deoxycholate; control titer >107, after-treatment titer 102.