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Laboratory-reared Aedes taeniorhynchus and Aedes sollicitans were readily infected with Cache Valley virus after ingestion of artificial mixtures of virus and defibrinated rabbit blood. Whereas some mosquitoes were infected with as little as three infective doses, 13 to 30 doses regularly infected all mosquitoes. Evidence for proliferation of ingested virus was obtained for both mosquito species; virus increased 100- to 500-fold over 10 to 14 days of extrinsic incubation. Infected mosquitoes regularly transmitted the virus to suckling mice by bite 6 to 10 days after feeding on infective blood.
Present address: Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin.
Present address: Entomology Research Division, Agricultural Research Service, U. S. Dept. of Agriculture, Beltsville, Maryland 20705.