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Preliminary observations indicating that cattle resident in coastal Maryland and Virginia are natural hosts for Cache Valley virus were confirmed by extended serologic surveys. That virus-neutralizing substances in cattle were indeed antibody was established by demonstration that serum antiviral activity resided in bovine immunoglobulins. Agespecific antibody prevalence was highest in cattle reared in coastal areas, and declined in piedmont or mountainous regions. In 1964 and 1965 about 50% of susceptible cattle of nine dairy herds in the endemic area became infected.
Present address: Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin 53706.
Present address: Microbiological Associates, Bethesda, Maryland 20014.