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Ultrastructure of Entamoeba Histolytica Trophozoites Obtained from the Colon and from in Vitro Cultures
Weleed El-Hashimi
Weleed El-Hashimi
Gastroenterology Research Laboratories, Department of Medicine, Tulane University, New Orleans, Louisiana 70112
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Fred Pittman
Fred Pittman
Gastroenterology Research Laboratories, Department of Medicine, Tulane University, New Orleans, Louisiana 70112
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The structure of Entamoeba histolytica trophozoites obtained by aspiration from the human colon and by cultivation in vitro was examined with the electron microscope. Amebae from both sources contained poorly developed endoplasmic reticulum, numerous vacuoles, glycogen particles, small cylindrical electron-dense bodies, and crystalloid structures within the cytoplasm. A well-defined fuzzy coat was present on amebae from the colon. A specific mechanism for the extracellular release of enzymes was not observed. In cultures, a number of dead amebae, some engulfed by living amebae, were seen. It is postulated that lytic enzymes responsible for the pathogenic activity of E. histolytica are released only after the death of some amebae in the colony.
Author Notes
Present address: 1/1/7 Karradat Mariam, Baghdad, Iraq.
Present address: Department of Medicine, Medical University of South Carolina, Charleston, South Carolina 29401.
| Past two years | Past Year | Past 30 Days | |
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| PDF Downloads | 9 | 6 | 0 |