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Purification of Complement-Fixing Antigens of Rickettsia Orientalis by Ether Extraction
Yuzuru Kobayashi
Yuzuru Kobayashi
Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
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Kunio Nagai
Kunio Nagai
Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
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Nobuyashi Tachibana
Nobuyashi Tachibana
Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
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Soluble and particulate antigens of Rickettsia orientalis were obtained by a single preparation of ether extraction of yolk-sac suspension previously treated with Amberlite XE-64. Cross-complement-fixation tests indicated that the soluble antigen has groupor species-specificity and the particulate antigen has type- or strain-specific activity. The soluble antigen was heat-labile, whereas the particulate antigen was heat-stable. Both the soluble and particulate antigens resisted trypsin and phosphatide acyl-hydrolase (phospholipase A). The soluble antigen was partially inactivated by periodic oxidation, but the particulate antigen was resistant to periodate. No change in complement-fixing titers was observed after lyophilization of the antigens. Wassermann-positive serum with high titer showed falsely positive reactions against normal yolk-sac and infected yolk-sac antigens.
| Past two years | Past Year | Past 30 Days | |
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