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In the course of arbovirus studies in the Ohio-Mississippi Basin, trivittatus virus was isolated from Aedes trivittatus mosquitoes, and a strain of Western equine encephalomyelitis virus (WEE) was isolated from the brain of a horse. Surveys for the presence of trivittatus neutralizing antibody resulted in the detection of protective serum from human beings, dogs, and cottontail rabbits. Although epizootics due to WEE virus had recently involved equine populations, there was little serologic evidence to indicate that human residents of the area had been infected.