A Comparison of Several Methods for Preparing Arbovirus Hemagglutinating and Complement-Fixing Antigens

Frank A. Pedreira National Institutes of Health, Division of Biologics Standards, Laboratory of Virology & Rickettsiology, Arbovirus Unit, and National Institute of Child Health and Human Development, Bethesda, Maryland 20014

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Nicola M. Tauraso National Institutes of Health, Division of Biologics Standards, Laboratory of Virology & Rickettsiology, Arbovirus Unit, and National Institute of Child Health and Human Development, Bethesda, Maryland 20014

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Michael J. Klutch National Institutes of Health, Division of Biologics Standards, Laboratory of Virology & Rickettsiology, Arbovirus Unit, and National Institute of Child Health and Human Development, Bethesda, Maryland 20014

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Alexis Shelokov National Institutes of Health, Division of Biologics Standards, Laboratory of Virology & Rickettsiology, Arbovirus Unit, and National Institute of Child Health and Human Development, Bethesda, Maryland 20014

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Five methods for preparing arbovirus hemagglutinating (HA) and complement-fixing (CF) antigens were compared. The antigen preparations included: sucrose-acetone (SA)-extracted suckling-mouse brain (SMB), crude SMB, Genetron (Gen)-extracted SMB. 20 × concentrated virus-infected cell cultures, and the supernatant fluid of 20 × concentrated cell cultures. Our results demonstrated that adequate arbovirus HA antigens can be made simply by Gen extraction of SMB suspensions. The advantages of this procedure over the more laborious, conventional SA-extraction procedure are emphasized. We were unable to prepare satisfactory HA antigens from cell cultures. Adequate CF antigens were readily prepared from 20 × concentrates of virus-infected cell cultures. crude and Genextracted SMB suspensions. The advantages to arbovirus serologists of the various methods are discussed.

Author Notes

Present address: Department of Microbiology, The University of Texas Medical School at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229.

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