Twelve arbovirus isolations were made from about 50,000 bloodsucking Diptera, including Culicidae (mosquitoes). Tabanidae (horseflies and deerflies), Simuliidae (black flies), and Ceratopogonidae (punkies) that were collected during 1965 in Wisconsin. The Jamestown Canyon serotype of the California virus complex was isolated from two species of Tabanidae, the horsefly, Hybomitra lasiophthalma (Macq.), and the deerfly Chrysops cincticornis (Walk.), and from the Aedes stimulans (Walk,) and the Aedes communis (DeG,) groups of mosquitoes. The virus apparently circulated among insects for only a short period (June) during 1965. Other isolations in 1965 included trivittatus virus from Aedes trivittatus and as yet unidentified arboviruses from a black fly. Simulium meridionale Riley, and a mixed pool containing mostly Culicoides.
Present address: Department of Bacteriology and Parasitology, Faculty of Veterinary Medicine, Aristotelian University, Thessaloniki, Greece.