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Tensaw virus, a member of the Bunyamwera arbovirus group, was isolated on 156 occasions from 365,654 mosquitoes tested between 1960–1963 from southwest Alabama, southeast Georgia, and central and south Florida. Of these isolates, 116 were from Anopheles crucians Wiedemann and 31 from Psorophora confinnis (Lynch-Arribalzaga); the remaining nine were from five other mosquito species, including Anopheles quadrimaculatus Say, Aedes atlanticus Dyar & Knab, A. mitchellae (Dyar), Culex nigripalpus Theobald, and Mansonia perturbans (Walker). Antibodies were detected in dogs, raccoons, cattle, and man; also the virus was isolated from the blood of a dog. No evidence of infection was noted in wild birds or in sentinel chickens.