Specific high-titered arbovirus CF antibody was obtained in mouse ascitic fluid. Primary and secondary immunization consisting of multiple closely spaced injections produced an antibody response similar to that found after one primary and one secondary injection, but resulted in higher-titered yet similarly specific reagents. Subcutaneous inoculation was shown to be more effective than intraperitoneal inoculation for primary immunization, whereas the reverse was true for secondary immunization. CF and HI antibody in hyperimmune ascitic fluid was found only in the 7S gamma G-immunoglobulin region. AC activity was found in the 19S portion of the gamma globulin region of unheated ascitic fluid; it was heat labile when isolated from 7S gamma G-immunoglobulins and heat stable in their presence. Nonspecific inhibitors in the HI test were shown to be lipoproteins that were not associated with 19S or 7S globulins.
Department of Virus Diseases, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012.
Department of Microbiology, University of Maryland, College Park, Maryland 20740.