By Everard L. Napier, M.R.C.S., L.R.C.P. (Lond.). In charge Kala-azar research, Calcutta School of Tropical Medicine. Second edition. 185 pages of text with 15 charts in the text, 18 plates, and an appendix of references to literature, author index and subject index. Oxford University Press. London, Bombay, Calcutta, Madras, 1927
A number of monophasic and diphasic media, with different gas phases and pH ranges, were used in the cultivation of Mesocestoides tetrathyridia in vitro. Growth and multiplication were evaluated by determining increases in the number of organisms and increases in protein after different periods of culture. In diphasic media containing whole human blood, asexual multiplication in vitro occurred consistently at a rate comparable to that in the mouse host. Good growth and some multiplication was obtained in medium containing human plasma.