By Richard C. Holcomb, M.D., F.A.C.S., Captain, Medical Corps, U. S. Navy, Retired. With Introduction by C. S. Butler, A.B., M.D., Li.D., Rear Admiral, Medical Corps, U. S. Navy. Pp. 1-189. Froben Press. New York. 1937
A number of monophasic and diphasic media, with different gas phases and pH ranges, were used in the cultivation of Mesocestoides tetrathyridia in vitro. Growth and multiplication were evaluated by determining increases in the number of organisms and increases in protein after different periods of culture. In diphasic media containing whole human blood, asexual multiplication in vitro occurred consistently at a rate comparable to that in the mouse host. Good growth and some multiplication was obtained in medium containing human plasma.