Isolation of Japanese Encephalitis Virus from the Blood of a Child in Singapore

Y. C. ChanDepartment of Bacteriology and Department of Pediatrics, University of Singapore, Singapore

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T. F. LohDepartment of Bacteriology and Department of Pediatrics, University of Singapore, Singapore

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Summary

The paper describes the clinical history and the isolation of a strain of Japanese encephalitis virus from the blood of a child. The virus was successfully re-isolated from the original acute phase serum and identified by complement-fixation, neutralization and hemagglutination inhibition antibody absorption tests. The etiological relationship of the virus to the patient's illness was established by the demonstration of complement-fixing, hemagglutination inhibition and neutralizing antibodies to the virus in the convalescent phase serum.

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