Isolation and Characterization of Arboviruses from Almirante, Republic of Panama

Pauline H. Peralta Middle America Research Unit, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Balboa Heights, Canal Zone

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Alexis Shelokov Middle America Research Unit, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Balboa Heights, Canal Zone

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John E. Vogel Middle America Research Unit, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Balboa Heights, Canal Zone

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Dorothy Longfellow Middle America Research Unit, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Balboa Heights, Canal Zone

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Summary

During the 22 months of a collaborative study carried out in Almirante, Panama, 1,341 pools comprising 181,979 mosquitoes and Phlebotomus sandflies were inoculated into suckling mice and hamster kidney cell cultures. Thirty-nine viruses were thus isolated which were classified as 9 types of 5 recognized groups, and one previously undescribed, totally distinct agent. Previously unknown on the Isthmus of Panama were: Una virus of group A, Guaroa, Cache Valley and Wyeomyia-complex viruses of the Bunyamwera group, and a member of the Guama group. The most noteworthy finding was the repeated isolation from Phlebotomus sandflies of Indiana type vesicular stomatitis virus and of the apparently new agent.

Author Notes

Present address: Laboratory of Virology and Rickettsiology, DBS, Bldg. 29, Rm. 316, National Institutes of Health, Bethesda, Md. 20014.

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